| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134598 |
| Chromosome: | chromosome 2 |
| Location: | 6021534 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112400 | CHI1 | (1 of 1) K12310 - Di-N-acetylchitobiase (CTBS); Chitinase-like hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCGGCCCATGGCACGTCCACGCTAATC |
| Internal bar code: | TGGATGTCGATATAGCTCCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 99.8468 |
| LEAP-Seq n confirming: | 4561 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAACAGCACTATCGCAT |
| Suggested primer 2: | CAAGCCGAATCACAGCACTA |