| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134611 |
| Chromosome: | chromosome 16 |
| Location: | 371114 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693750 | (1 of 1) K11462 - polycomb protein EED (EED) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTGCCGTCGCCGTCGGCGCCGCCGCCC |
| Internal bar code: | GATCTACAGTACCAGCATGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 58.2701 |
| LEAP-Seq n confirming: | 384 |
| LEAP-Seq n nonconfirming: | 275 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAAGTTGTGCCTGCGTCT |
| Suggested primer 2: | GTGTGGCCTTCAACACCTTT |