Insertion junction: LMJ.RY0402.134621_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.134621
Chromosome:chromosome 6
Location:1950416
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g263750 RPN15 component of lid subcomplex of 26S proteasome regulatory subunit; (1 of 2) PF05160 - DSS1/SEM1 family (DSS1_SEM1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTGATTACTGCGCGCCAGGATCGGGAGC
Internal bar code:CGAGTATATGTGGCCGCGGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:991
LEAP-Seq percent confirming:99.9152
LEAP-Seq n confirming:8245
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACACCAAATCGTCTTCT
Suggested primer 2:CTGCCTTACCACTGTCCCAT