| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134626 |
| Chromosome: | chromosome 9 |
| Location: | 1751380 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGATTGAGGTAACTGGGTTGCCAGGGAT |
| Internal bar code: | AGTTTTGTTGTAGCAGATCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 674 |
| LEAP-Seq percent confirming: | 99.3616 |
| LEAP-Seq n confirming: | 4358 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAATTGCGTATGGCCTTT |
| Suggested primer 2: | ATTCGTCCACAATCGACACA |