Insertion junction: LMJ.RY0402.134634_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.134634
Chromosome:chromosome 7
Location:2533655
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g329750 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCGGCCATCGCCGCTACTGCGCACAGA
Internal bar code:CCCGCGCCCCGGGAGCAGGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:613
LEAP-Seq percent confirming:98.8608
LEAP-Seq n confirming:13625
LEAP-Seq n nonconfirming:157
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:AGTATGGACACACGCACGTC
Suggested primer 2:ATGTCAGATGAGCACGACGA