| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134641 |
| Chromosome: | chromosome 10 |
| Location: | 1821416 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431100 | OPR126 | OctotricoPeptide Repeat protein 126 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCGTTCCGCTACGTGGCGGAGCTTCGG |
| Internal bar code: | GCCGCATCATTACGATGGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 76.6854 |
| LEAP-Seq n confirming: | 1092 |
| LEAP-Seq n nonconfirming: | 332 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTGTGGGTTGTGTGTGC |
| Suggested primer 2: | TTGGCAGGCATGTACTCAAG |