| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134675 |
| Chromosome: | chromosome 14 |
| Location: | 1108155 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615050 | (1 of 1) 1.3.1.38//1.3.1.93 - Trans-2-enoyl-CoA reductase (NADPH) // Very-long-chain enoyl-CoA reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTAGTGGTATAGTGGTGGCTAGGATGGG |
| Internal bar code: | CGGGAGCAGGGGGGGGGTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 913 |
| LEAP-Seq percent confirming: | 99.8672 |
| LEAP-Seq n confirming: | 3761 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | TGTGATCAGCAACACAAGCA |