Insertion junction: LMJ.RY0402.134677_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.134677
Chromosome:chromosome 3
Location:2654587
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g160953 (1 of 1) 3.1.27.1//3.6.3.44 - Ribonuclease T(2) / Ribonuclease T2 // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTCGACGTGAGTGCCGGGGCTGCCCGC
Internal bar code:TGCTGGATATCGTCGCGCTCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:269
LEAP-Seq percent confirming:2.31056
LEAP-Seq n confirming:186
LEAP-Seq n nonconfirming:7864
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTGTGTGTGTGTGTGTGT
Suggested primer 2:TTTTCTCTCCTCACCTGCGT