| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134721 |
| Chromosome: | chromosome 9 |
| Location: | 3539737 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389912 | (1 of 1) K14018 - phospholipase A-2-activating protein (PLAA, DOA1, UFD3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCATCGCCGTGTGTGGTGTTTGGTATG |
| Internal bar code: | AGCAGATAGTGAACAGTGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 99.8295 |
| LEAP-Seq n confirming: | 2928 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTTTTCGTAATCCCCTTG |
| Suggested primer 2: | GTCTGTGTCGCAAGTCAGGA |