| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134724 |
| Chromosome: | chromosome 5 |
| Location: | 2047760 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241655 | (1 of 24) IPR001478 - PDZ domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGCTCAATAACGCTCGAGCGGTGGCCAT |
| Internal bar code: | TGTTAACGATCCCAATTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 99.7898 |
| LEAP-Seq n confirming: | 9493 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCTGATAGCTGTGTCCA |
| Suggested primer 2: | TGACCTGTGAGGAGCAACAG |