| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134725 |
| Chromosome: | chromosome 6 |
| Location: | 184822 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g250350 | (1 of 1) PF05216 - UNC-50 family (UNC-50) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCGTGTTGTCGGCGGTGCTGGTTGACTT |
| Internal bar code: | ACCTGGCTCAGTCCGTGTCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 650 |
| LEAP-Seq percent confirming: | 94.2766 |
| LEAP-Seq n confirming: | 1186 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTTGCTATAGCTTCACG |
| Suggested primer 2: | TGGCTATAATTGTGGCCTCC |