| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134753 |
| Chromosome: | chromosome 3 |
| Location: | 889315 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146627 | (1 of 1) PTHR20852:SF16 - TYPE-1 GLUTAMINE SYNTHETASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTGGCCATTCCCCTCCCACCCCGCCCCA |
| Internal bar code: | CCATCACCGTCACCACCCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 48 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTACTTGCCGCATATTTT |
| Suggested primer 2: | ATGCACCTTGACGTCCTACC |