| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134760 |
| Chromosome: | chromosome 11 |
| Location: | 1678162 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467778 | (1 of 1) K00761 - uracil phosphoribosyltransferase (upp, UPRT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTTGATTTAGTTTGGGCTGGTACCGG |
| Internal bar code: | GGCGCCACGTCAAAAGGAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 477 |
| LEAP-Seq percent confirming: | 99.875 |
| LEAP-Seq n confirming: | 3197 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATGAGGAACAGGTCGGT |
| Suggested primer 2: | TTCGCTTCTAAACCGCTCAT |