Insertion junction: LMJ.RY0402.134785_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.134785
Chromosome:chromosome 17
Location:181267
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g697406 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCGACATCGTCGCCGGCGCTGCCGGGTA
Internal bar code:GCGGTAGAGGTGTCGGCAGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:609
LEAP-Seq percent confirming:99.9234
LEAP-Seq n confirming:1305
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CATTCGCAGGATGATGTGAC
Suggested primer 2:GGAGCTCAAGCAGGGTGTAG