Insertion junction: LMJ.RY0402.134823_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.134823
Chromosome:chromosome 7
Location:4908923
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g346317 GT90F39,GT90-39 GT90 family protein 39; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAGTCCTCGTTGTACGACTTGCCGCCGG
Internal bar code:TATAGGCTTCCGCCCATGTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:631
LEAP-Seq percent confirming:99.4924
LEAP-Seq n confirming:980
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCCACACACATGCACACATA
Suggested primer 2:GGATGTTTGGAAAAGCGGTA