| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134832 |
| Chromosome: | chromosome 6 |
| Location: | 1819248 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262650 | OPR22,TAA1 | (1 of 66) 2.7.12.1 - Dual-specificity kinase; Translation factor for psaA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCGCCTGCTTGCCGCTCGGAGCTGGCTTC |
| Internal bar code: | TGGGCTAGGGAGAGTTGGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 45.6934 |
| LEAP-Seq n confirming: | 313 |
| LEAP-Seq n nonconfirming: | 372 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGAACTCCACCCAGTCC |
| Suggested primer 2: | CACTTGCTCCGCTTCTTCTC |