| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134836 |
| Chromosome: | chromosome 11 |
| Location: | 2744959 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477150 | (1 of 4) IPR000533 - Tropomyosin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCGCGGACCGGCAGCTGCAGGACCTGC |
| Internal bar code: | GTATCATTCTATCCATTGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 68 |
| LEAP-Seq percent confirming: | 99.2593 |
| LEAP-Seq n confirming: | 134 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACTGGGAATGGTTCTAA |
| Suggested primer 2: | CATGTCGTCCTGCGTCTCTA |