| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134862 |
| Chromosome: | chromosome 15 |
| Location: | 1701736 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g643750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGACAATAACGACAGTGGCAAACGAATAA |
| Internal bar code: | GTGCCGGCAGACGAGCATTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 97.0803 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTAGCAGTTCGGGTCAGG |
| Suggested primer 2: | ATGCCGCAGAAAATAACCAC |