Insertion junction: LMJ.RY0402.134877_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.134877
Chromosome:chromosome 6
Location:5457021
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g284400 (1 of 1) K06695 - 26S proteasome regulatory subunit, ATPase 3, interacting protein (PSMC3IP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACCCACATAGACACAAGGCGATGCCAG
Internal bar code:CAGATGCTCCCTTGGACGTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:167
LEAP-Seq percent confirming:98.8889
LEAP-Seq n confirming:89
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGTGGAACATGAAAGGTG
Suggested primer 2:GCAAGGACAGATGACTGCAA