| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134881 |
| Chromosome: | chromosome 2 |
| Location: | 2885682 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094900 | (1 of 1) PTHR23083//PTHR23083:SF427 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTGTTGAGTATACTGCCCCCCATAAG |
| Internal bar code: | CACAGACCCCATTAGATCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 408 |
| LEAP-Seq percent confirming: | 95.3347 |
| LEAP-Seq n confirming: | 470 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTCCTTTTGAGCGTTTC |
| Suggested primer 2: | CAATCGCACGTATGGTATGC |