Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.134890 |
Chromosome: | chromosome_13 |
Location: | 3742904 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589450 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCAGCCACAACTCGCTGTACAGCAACTGCA |
Internal bar code: | GACGGCCAAATTGGAATTGGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 690 |
LEAP-Seq percent confirming: | 99.0738 |
LEAP-Seq n confirming: | 2995 |
LEAP-Seq n nonconfirming: | 28 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACGGTGGAGTTCAGCTACC |
Suggested primer 2: | CAGGCTCAGAGCTAACCACC |