| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134904 |
| Chromosome: | chromosome 9 |
| Location: | 4663143 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396735 | (1 of 1) PTHR18895:SF3 - METHYLTRANSFERASE-LIKE PROTEIN 20 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACAGCAGTCGTGCCTGTACGACATGCAT |
| Internal bar code: | GAGGCACCGGATAGAACCCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 99.7215 |
| LEAP-Seq n confirming: | 10024 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTTGACCACGAAGTCCT |
| Suggested primer 2: | CGTGAAATGGCGAGTGTATG |