Insertion junction: LMJ.RY0402.134910_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.134910
Chromosome:chromosome 8
Location:1896563
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g367150 MFT23 Major facilitator superfamily transporter; (1 of 22) IPR011701//IPR020846 - Major facilitator superfamily // Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACCAGGGGCGGGGGAAGCGGTTGTCCA
Internal bar code:ACCTTGGTGCCACCGTAGCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:54
LEAP-Seq percent confirming:99.8088
LEAP-Seq n confirming:522
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGTCATCCCGTGTCATC
Suggested primer 2:AATCGGTGAACGACCAAGTC