| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134911 |
| Chromosome: | chromosome 16 |
| Location: | 2308731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659250 | CYB5,CYB5-5 | (1 of 2) PTHR19359//PTHR19359:SF29 - CYTOCHROME B5 // SUBFAMILY NOT NAMED; Cytochrome b5 flavohemoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGTGGGGGAGATGTAAGGTGCGGCGT |
| Internal bar code: | GTTGCGGCGCCGGACTAGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 927 |
| LEAP-Seq percent confirming: | 99.7803 |
| LEAP-Seq n confirming: | 5905 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTGGACCTGTGGTTGGT |
| Suggested primer 2: | GGAGCTTGAGTCCTCCTGTG |