| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134921 |
| Chromosome: | chromosome_17 |
| Location: | 6206522 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g741950 | BBS1 | Bardet-Biedl syndrome-1 associated protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGTTCGGTTCAAGCGTCCCCTGGGCCTGCT |
| Internal bar code: | TGGGTTTAGTTGTTTATTTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 94.386 |
| LEAP-Seq n confirming: | 269 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTGTGAAGTTGGTGGGG |
| Suggested primer 2: | AATGGTTTAGGTGCTGGTGC |