| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134924 |
| Chromosome: | chromosome 1 |
| Location: | 3878306 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025200 | MMP4 | Metalloproteinase of VMP family; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATTAGTCGGGAAGCTTATTTCATATAC |
| Internal bar code: | TTCAGGGACCGTCCTAGCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 85.9885 |
| LEAP-Seq n confirming: | 448 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAATGAACGGTGTGACG |
| Suggested primer 2: | ACCTCGCATAACCACAGGAC |