| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134925 |
| Chromosome: | chromosome 1 |
| Location: | 601659 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003475 | (1 of 2) 3.4.24.35 - Gelatinase B / Type V collagenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTGGTTGGCGATGGCATGGCGGGCGCT |
| Internal bar code: | TGCTTGACAGTGACCAGTTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 536 |
| LEAP-Seq percent confirming: | 99.6315 |
| LEAP-Seq n confirming: | 11084 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAACACATGCTCCCAGTC |
| Suggested primer 2: | GCATCCCCCACTTTCAGTTA |