Insertion junction: LMJ.RY0402.134926_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.134926
Chromosome:chromosome 17
Location:891355
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g702700 TPT28,TPT27 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 1) PTHR11132//PTHR11132:SF101//PTHR11132:SF125 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCGTGCTGCGTGGCACCCAAGGCACGG
Internal bar code:GGGCCCGTTCGTTCTTGCCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:301
LEAP-Seq percent confirming:99.1778
LEAP-Seq n confirming:1930
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTTTCAATGCCTCCTTC
Suggested primer 2:GCTGTTTTGAGGAAACTCGC