| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134970 |
| Chromosome: | chromosome 1 |
| Location: | 4499519 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030650 | PHR5,CRY-DASH2,DCRY2 | (1 of 3) K01669 - deoxyribodipyrimidine photo-lyase [EC:4.1.99.3] (phrB); DASH-type Cryptochrome 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCCCACCACCTCCCTGTCCGGACCCCGG |
| Internal bar code: | CGCGGAACATGTGCCGGCATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 954 |
| LEAP-Seq percent confirming: | 76.8925 |
| LEAP-Seq n confirming: | 4642 |
| LEAP-Seq n nonconfirming: | 1395 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGTGGGCTGACCTTAG |
| Suggested primer 2: | AGTGTTCATGGAAGGCAACC |