| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134972 |
| Chromosome: | chromosome_9 |
| Location: | 2224445 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392900 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGCTGGGTGGAGATGAGGAGATTGACCA |
| Internal bar code: | GCAAAAGGGCCGTAGGAGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 99.0748 |
| LEAP-Seq n confirming: | 7389 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAGATGATGGATTCGGC |
| Suggested primer 2: | GCAGACAGCAATGATCTGGA |