| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134999 |
| Chromosome: | chromosome 2 |
| Location: | 5738126 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110300 | DNJ25 | (1 of 62) PF00226 - DnaJ domain (DnaJ); DnaJ-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTGTGCAAGATTGTGGGTCTGACAACTC |
| Internal bar code: | CCTATCCAAGAGCTCCAGGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 96.4973 |
| LEAP-Seq n confirming: | 12535 |
| LEAP-Seq n nonconfirming: | 455 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATCAAGGCCGCTTAGTGC |
| Suggested primer 2: | AGTCACTGCTGCATGTACGG |