| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135021 |
| Chromosome: | chromosome 2 |
| Location: | 1571086 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g084873 | (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTTCCCGTCGGCTGTGGCTGCAGGCAC |
| Internal bar code: | ACTCGTCCAATTGACTACGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 91.6129 |
| LEAP-Seq n confirming: | 142 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCCTGCGATGTGTAGAA |
| Suggested primer 2: | GTTGACATATGGCGAGAGCA |