| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135029 |
| Chromosome: | chromosome 9 |
| Location: | 4500958 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395806 | (1 of 5) 1.14.13.114 - 6-hydroxynicotinate 3-monooxygenase / HNA-3-monooxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGGCAAGGGGCAGGGGTTTCGAAATGT |
| Internal bar code: | AAAAGACGTGTGTGGTTAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 99.4885 |
| LEAP-Seq n confirming: | 778 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTTTCCTGCCTCTTACA |
| Suggested primer 2: | AGTCCTTACATGTCACCCGC |