| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135046 |
| Chromosome: | chromosome 11 |
| Location: | 604120 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467611 | (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCACCAGGTCACTGAACCGCGCCCGC |
| Internal bar code: | ACGCGTGCATCTACAAGTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 205 |
| LEAP-Seq percent confirming: | 23.933 |
| LEAP-Seq n confirming: | 2086 |
| LEAP-Seq n nonconfirming: | 6630 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCTTGCTCGGTTGTTC |
| Suggested primer 2: | CCTAAAATGCCTGTCCCAGA |