| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135049 |
| Chromosome: | scaffold 18 |
| Location: | 231822 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749797 | FAP281 | Flagellar Associated Coiled-Coil Protein 281 | CDS/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCGCGCTAACCCGCGCGAGGCCGCGCA |
| Internal bar code: | ATTCAATTCTGGGTTAAGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 99.1264 |
| LEAP-Seq n confirming: | 3177 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTGCCCATCCAGATACT |
| Suggested primer 2: | CGTCCATGACCTAGAGGGAA |