| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135056 |
| Chromosome: | chromosome 17 |
| Location: | 2099981 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712250 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCGAGCAGAAGATGCTCCGTCTTCAGT |
| Internal bar code: | GTGAGGGTCACTGGTTGCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 937 |
| LEAP-Seq percent confirming: | 99.101 |
| LEAP-Seq n confirming: | 3307 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGCAGAGCAAATCCTTG |
| Suggested primer 2: | GAGCTGGACTCTTTGCATCC |