| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135069 |
| Chromosome: | chromosome_4 |
| Location: | 1237446 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g215250 | FAP26 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACTTGAGCGCCTTGATGGCCGCAGTCACCA |
| Internal bar code: | CACAGGCGCGAGGAGCAATCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 94.9782 |
| LEAP-Seq n confirming: | 435 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCAATCTATCCGCTCAC |
| Suggested primer 2: | TTTGCTCGTGAAGGGAGAGT |