Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.135073 |
Chromosome: | chromosome 9 |
Location: | 5248136 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g399626 | (1 of 1) K09919 - hypothetical protein (K09919) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTGCCGCTACCGTACTCGCATGCCTCT |
Internal bar code: | TGAAAATACAAGCGTGGGGCCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 378 |
LEAP-Seq percent confirming: | 99.25 |
LEAP-Seq n confirming: | 397 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGGTTTGTATAGGGCGTAA |
Suggested primer 2: | TATGGACAGGCACTGGATGA |