| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135078 |
| Chromosome: | chromosome 1 |
| Location: | 7254268 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052300 | E2F1 | (1 of 1) K06620 - transcription factor E2F3 (E2F3); Transcription factor, E2F and DP-related | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGGGTTGATAACGACCTTTTCCTGCC |
| Internal bar code: | AGTACCAGGTAGTATATCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 805 |
| LEAP-Seq percent confirming: | 99.8654 |
| LEAP-Seq n confirming: | 742 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACGGTATCCGTGTGCTG |
| Suggested primer 2: | TTGGTGCTCACATCAAGAGC |