Insertion junction: LMJ.RY0402.135079_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.135079
Chromosome:chromosome 7
Location:1611602
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g325000 CYP18,CYP738A1 Cytochrome P450, CYP120 superfamily; (1 of 4) 1.14.13.93 - (+)-abscisic acid 8'-hydroxylase / ABA 8'-hydroxylase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAGCTCCGCAATGGCTGGCACGTGCCG
Internal bar code:TCCGCACTCTAGACGGGGAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:790
LEAP-Seq percent confirming:99.4844
LEAP-Seq n confirming:5788
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AGACCACTCGGTCAAACACC
Suggested primer 2:GGTGAAACGTTGGAGCTCAT