Insertion junction: LMJ.RY0402.135089_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.135089
Chromosome:chromosome 9
Location:7680380
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g415500 TCA1 Translation factor for chloroplast petA RNA 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCGTCTGATAGCAGCCGAACTAACTAGC
Internal bar code:AAGGTCCGGGCGGAATCCGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:399
LEAP-Seq percent confirming:99.666
LEAP-Seq n confirming:2089
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CAATGGTTTGGGCTTCAAGT
Suggested primer 2:CTGTCTGCAGGTGCTGCTAC