| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135093 |
| Chromosome: | chromosome 16 |
| Location: | 3746653 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689150 | SQD3,GTR23 | Sulfoquinovosyldiacylglycerol synthase; (1 of 2) K06119 - sulfoquinovosyltransferase (SQD2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTTGTCAGCCTGCTGGTCGGCGTAGCT |
| Internal bar code: | GCAGTCCGCCGCGGGCGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 98.8691 |
| LEAP-Seq n confirming: | 612 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGACAGCAACCCGAGTC |
| Suggested primer 2: | GTCGTGTATGACTGGGCCTT |