Insertion junction: LMJ.RY0402.135105_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.135105
Chromosome:chromosome 2
Location:7346778
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g145100 FAP39 Flagellar Associated Protein 39; (1 of 1) PF00122//PF00689//PF00690//PF00702//PF12710 - E1-E2 ATPase (E1-E2_ATPase) // Cation transporting ATPase, C-terminus (Cation_ATPase_C) // Cation transporter/ATPase, N-terminus (Cation_ATPase_N) // haloacid dehalogenase-like hydrolase (Hydrolase) // haloacid dehalogenase-like hydrolase (HAD) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTACCCCGAGCTGCTGGACGAGATGCCA
Internal bar code:ATGAGTCAGGCAGCACAGAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:923
LEAP-Seq percent confirming:61.2266
LEAP-Seq n confirming:3504
LEAP-Seq n nonconfirming:2219
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CAACTTCAGCTCCATCGTCA
Suggested primer 2:CCGCAGTAGCCCATAATGTT