| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135112 |
| Chromosome: | chromosome 16 |
| Location: | 1524879 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g653300 | BLZ19 | bZIP transcription factor; (1 of 21) IPR004827 - Basic-leucine zipper domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCCTGATCGGGCTCGCATTCTAGTCGG |
| Internal bar code: | CCTATCAGTCGTCCCCAGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 589 |
| LEAP-Seq percent confirming: | 99.425 |
| LEAP-Seq n confirming: | 1729 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTTCCAGGGAAGAGGACA |
| Suggested primer 2: | TGCAGGAGACGCTTAGGATT |