| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135119 |
| Chromosome: | chromosome 10 |
| Location: | 4665146 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453250 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCCCCTGTTGAAGTCGGGGCGAGTGCC |
| Internal bar code: | GTCGAACGGTGCCGGAGGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1041 |
| LEAP-Seq percent confirming: | 96.9325 |
| LEAP-Seq n confirming: | 790 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGGGGATTCGTGTTTA |
| Suggested primer 2: | GTGCATAGTTTGGGGCAGTT |