| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.135123 |
| Chromosome: | chromosome 7 |
| Location: | 1066984 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319650 | FXL4,FXL5 | FixL-like PAS domain protein; (1 of 3) IPR000014//IPR013767 - PAS domain // PAS fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGAGTTAAACGAGGCAAGCGCCGTCAG |
| Internal bar code: | GAGTGGTGAGACCCTCCTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 17.8625 |
| LEAP-Seq n confirming: | 1190 |
| LEAP-Seq n nonconfirming: | 5472 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTACTCGCCTCGATACGC |
| Suggested primer 2: | AAGTACCCACGCGATACAGG |