| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.135124 |
| Chromosome: | chromosome 2 |
| Location: | 953810 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079850 | POLK1 | (1 of 1) K03511 - DNA polymerase kappa (POLK); DNA polymerase kappa | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGAATCCCACTTCGTATTGCATTATTGG |
| Internal bar code: | AGACCCATGTTCTGCTTTGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 99.7352 |
| LEAP-Seq n confirming: | 1883 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCACGTTCTGGTGATAGT |
| Suggested primer 2: | TGCAACTTGGGTGTGCTTAG |