Insertion junction: LMJ.RY0402.135128_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.135128
Chromosome:chromosome 12
Location:4826334
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g524650 HDA18,SRTB1,SRTB (1 of 1) K11414 - NAD-dependent deacetylase sirtuin 4 [EC:3.5.1.-] (SIRT4, SIR2L4); Sir2-like NADH dependent histone deacetylase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCCATCTACCTGCACCGCGCGTCGGCCC
Internal bar code:GTAATGTAGTGACGGGCTCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:342
LEAP-Seq percent confirming:99.5833
LEAP-Seq n confirming:239
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAACATGCGCTTACATCCAC
Suggested primer 2:GTACCGGCAGTAGAAGCAGC