| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.135144 |
| Chromosome: | chromosome 1 |
| Location: | 5143960 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035800 | IRK2 | (1 of 2) PTHR11767//PTHR11767:SF47 - INWARD RECTIFIER POTASSIUM CHANNEL // SUBFAMILY NOT NAMED; Inwardly-rectifying potassium channel | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACGCAACGCCTCCAAAATCTTCCCAAG |
| Internal bar code: | CGCCTTGGAGTGGCTCCGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 78.3237 |
| LEAP-Seq n confirming: | 271 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGAGAGCAGACTACACG |
| Suggested primer 2: | ACTGGACCGACATCTTCCAC |