| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.135164 |
| Chromosome: | chromosome 10 |
| Location: | 4900449 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454700 | (1 of 1) IPR001611//IPR003591//IPR025875 - Leucine-rich repeat // Leucine-rich repeat, typical subtype // Leucine rich repeat 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATTGGCGCGGTTCCTTCAGCTGGACCTG |
| Internal bar code: | TCGGGGATAAGCATGCGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 99.0693 |
| LEAP-Seq n confirming: | 2874 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTTGAGCACCTTGAGGC |
| Suggested primer 2: | CTGTTCGAGTGAGGAGGGAG |